Multiple Forms of Flounder Muscle Glyceraldehyde 3-Phosphate Dehydrogenase
نویسندگان
چکیده
منابع مشابه
Tissue-characteristic forms of glyceraldehyde 3-phosphate dehydrogenase.
Glyceraldehyde 3-phosphate dehydrogenase exists in two different forms in various human tissue preparations. One of them is exhibited, after starch-gel electrophoresis, by a rapidly migrating or ;fast' band and the other by a ;slow' band. The proportion of the total activity in each of the two forms is characteristic of the type of tissue. A particulate fraction, obtained after centrifugation o...
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The reaction catalyzed by this enzyme consists in the oxidation of a -CHO to a -COO. POSH2 group and represents the first oxidative step in the degradation of carbohydrate in the tissues. Diphosphopyridine nucleotide (DPN) and inorganic phosphate are necessary for this reversible enzymatic reaction. The equilibrium has been investigated by Warburg and Christian (l), Drabkin and Meyerhof (2), an...
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The catalytic interaction of glyceraldehyde-3-phosphate dehydrogenase with glyceraldehyde 3-phosphate has been examined by transient-state kinetic methods. The results confirm previous reports that the apparent Km for oxidative phosphorylation of glyceraldehyde 3-phosphate decreases at least 50-fold when the substrate is generated in a coupled reaction system through the action of aldolase on f...
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Glyceraldehyde-3-phosphate dehydrogenase catalyzes the oxidative phosphorylation of n-glyceraldehyde-3-phosphate to 1,3-diphosphoglyceric acid (1, 2). The enzyme also catalyzes the oxidation of n-glyceraldehyde, although the product of this reaction has not been characterized (1,2). In the present study it is shown that acetaldehyde (3), propionaldehyde, and butyraldehyde also act as substrates...
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The exceptionally high protein concentration in living cells can favor functional protein-protein interactions that can be difficult to detect with purified proteins. In this study we describe specific interactions between mammalian D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and L-lactate dehydrogenase (LDH) isozymes from heart and muscle. We use poly(ethylene-glycol) (PEG)-induced copr...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43023-8